faps (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems faps
Faps, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+fap+antibody/Human+PDGF+R+alpha+Antibody/pmc13082186-78-68-75
Average 93 stars, based on 79 article reviews

faps - by Bioz Stars, 2026-10

93/100 stars

Images

Incubation:

Article Title: Nanobody-based trispecific T cell engager (Nb-TriTE) enhances therapeutic efficacy by overcoming tumor-mediated immunosuppression
Article Snippet: In addition, Nb-TriTE simultaneous binding ability to antigens was assessed by a two-step sandwich ELISA with hFAP, hCD3 epsilon, and hPD-1 (ACRO, FAP-H5263, CDE-H5256, PD1-H5257) as the capture reagents, biotinylated hFAP, biotinylated hCD3 epsilon, and biotinylated hPD-1 (ACRO, FAP-H82Q6, CDE-H82E1, PD1-H82E4) as the detection reagents, the OD 450 value was measured using a microplate reader (Tecan, Mannedorf, Switzerland). .. Incubation with a PE-conjugated anti-human FAP antibody (R&D Systems, USA) was performed to determine the expression of target cells, an APC-conjugated anti-human PD-L1 antibody (Biolegend, USA) was performed to determine the expression of target cells. .. Nb-TriTE (2 μg/mL) was used in conjunction with a PE-conjugated anti-His-tag antibody (R&D Systems, USA) and to analyze binding ability.

Article Title: Nanobody-based trispecific T cell engager (Nb-TriTE) enhances therapeutic efficacy by overcoming tumor-mediated immunosuppression.
Article Snippet: In addition, Nb-TriTE simultaneous binding ability to antigens was assessed by a two-step sandwich ELISA with hFAP, hCD3 epsilon, and hPD-1 (ACRO, FAPH5263, CDE-H5256, PD1-H5257) as the capture reagents, biotinylated hFAP, biotinylated hCD3 epsilon, and biotinylated hPD-1 (ACRO, FAP-H82Q6, CDE-H82E1, PD1-H82E4) as the detection reagents, the OD450 value was measured using a microplate reader (Tecan, Mannedorf, Switzerland). .. Incubation with a PE-conjugated anti-human FAP antibody (R&D Systems, USA) was performed to determine the expression of target cells, an APC-conjugated antihuman PD-L1 antibody (Biolegend, USA) was performed to determine the expression of target cells. .. Nb-TriTE (2 μg/mL) was used in conjunction with a PE-conjugated anti-His-tag antibody (R&D Systems, USA) and to analyze binding ability.

Expressing:

Control:

Article Title: Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intra-tumoral stromagenesis
Article Snippet: For IHC, the following antibodies were used: anti-human fibronectin (Sigma #F3648), anti-mouse versican (EMD Millipore #AB1033, Burlington, MA, USA), biotinylated hyaluronic acid binding protein, HABP (EMD Millipore #385911), Rabbit IgG isotype control (Santa Cruz Biotechnology #sc-2027, Dallas, TX, USA), and biotin-SP-conjugated goat anti-rabbit IgG secondary antibody (Jackson Immunoresearch #AB2337959, West Grove, PA, USA). .. The following antibodies were used for IF: anti-human FAP antibody (R&D Systems #AF3715, Minneapolis, MN, USA or Abcam #53066, Cambridge, MA, USA) or sheep IgG isotype control (R&D Systems #5001A), and AF488-conjugated donkey anti-sheep IgG secondary antibody (ThermoFisher #A11015, Waltham, MA, USA). .. All imaging was completed with the Olympus Spot-RT3 microscope at 20X magnification.

Article Title: Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model
Article Snippet: The anti- IL6, mAb (#MAB227) was obtained from R&D Systems GmbH, Wiesbaden-Nordenstadt, Germany. .. For flow cytometry, goat anti-human EpCAM/Trop-1 (# AF960), anti-human FAP antibody (# MAB3715), Isotype control antibodies (#AB-108-C and #MAB002) and the secondary antibodies, APC-labeled antibody for EpCAM (#F0108) and Alexa488-labeled antibody for FAP (#A21202) were purchased from R&D Systems GmbH, Wiesbaden-Nordenstadt, Germany. .. For Western blotting, the EGF Receptor (D38B1) XPRabbit mAb (#4267), the phospho-EGF Receptor (Tyr1068) antibody (#2234), the c-Met (L41G3) mouse mAb (#3148), the phospho-c-Met (Tyr1234/1235) (D26) XPRabbit mAb (#3077), the phospho-Stat3 (Tyr705) (D3A7) XPRabbit mAb (#9145), the Stat3 antibody (#9132) and the HRP-labeled anti-rabbit (#7074) and anti-mouse secondary antibodies (#7076) were all obtained from Cell Signaling Technology (New England Biolabs, Frankfurt am Main, Germany).

Flow Cytometry:

other:

Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug
Article Snippet: Anti-human FAP antibody (clone 427819; R&D Systems, Minneapolis, MN, USA), anti-PDPN antibody (clone 101AP; Life Technologies, Carlsbad, CA, USA), IRDye ® 700DX (IR700; Li-COR Bioscience, Lincoln, NE, USA), anti-calreticulin antibody-Alexa Fluor ® 488 (ab196158; Abcam, Cambridge, UK), Alexa Fluor ® 488 (Life Technologies), and gemcitabine hydrochloride (GEM; Eli Lilly, Indianapolis, IN, USA) were used.

Article Title: Treatment of Pancreatic Cancer Using Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts in Combination with Anticancer Chemotherapeutic Drug.
Article Snippet: Anti-human FAP antibody (clone 427819; R&D Systems, Minneapolis, MN, USA), anti-PDPN antibody (clone 101AP; Life Technologies, Carlsbad, CA, USA), IRDye® 700DX (IR700; Li-COR Bioscience, Lincoln, NE, USA), anti-calreticulin antibody-Alexa Fluor® 488 (ab196158; Abcam, Cambridge, UK), Alexa Fluor® 488 (Life Technologies), and gemcitabine hydrochloride (GEM; Eli Lilly, Indianapolis, IN, USA) were used.

Review